This product is used for lateral flow immunoassay to qualitatively detect nasopharyngeal and oropharyngeal swabs of patients with suspected infection.
[Sample requirements]
It is recommended to use the polyester sponge sample with PP (polypropylene) rod for aseptic sample collection.
(1) Collection method of oropharyngeal specimens: The collection head is slightly tilted, and both sides of pharyngeal tonsils are exposed in the mouth. Wipe the pharyngeal tonsil at least three times along the tongue root, and then wipe the posterior pharynx up and down with cotton swabs at least three times.
(2) Methods of collecting nasopharyngeal specimens: The sampler gently holds the recipient's head with one hand and the swab with the other hand. Insert the swab into the nostril and slowly insert it along the bottom of the inferior nasal meatus. Arc, don't use too much force to avoid bleeding due to trauma. When the tip of the swab reaches the back wall of the nasopharyngeal cavity, gently rotate once (if it is a reflex cough), and then slowly take out the swab.
(3) Sample treatment: the collected samples should be treated with the sample buffer provided by the kit as soon as possible (if not treated immediately, the samples should be stored in dry, sterilized and sealed plastic pipes), and stored at 2 C ~ 8 C, with long-term storage not exceeding 24 hours ~ -70°C, but repeated freezing and thawing should be avoided.
Test Method
Before testing, all reagents were returned to room temperature. The test should be carried out at room temperature.
I. sample extraction (see figure 1)
Add 400 L(~10 drops) of sample buffer vertically into the sample extraction tube, then insert the sample into the tube and rotate it against the inner wall for about 10 times, so that the sample can be dissolved in the solution as much as possible.
2. Sampling along the inner wall of the extraction tube, leaving as much liquid as possible in the tube, then taking it out and discarding it.
3. Cover the dropper.
Ii test procedure (see figure 2)
1. Take out the test card from the sealed bag.
2. Add 2 drops (~80 L) of processed sample extract to the sample well of the test card, and start the timer.
3. Place the test card at room temperature for 15 minutes and read the results. The result is invalid after 20 minutes.
Interpretation of test results
Schematic diagram of card result judgment:
Invalid (1) Result: The quality control line (line C) has no reaction line, and the test is invalid, so the test is repeated.
(2) Negative result: red ribbon, coloring of quality control line (line C).
(3) Positive results: two red bands, the detection line (T line) and the quality control line (C line) are colored.
